prl-cmv vectors (Promega)
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Promega
prl-cmv vectors
Prl Cmv Vectors, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prl-cmv+vector/prl+cmv+plasmid/pm40639948-317-5-10
Average 90 stars, based on 1 article reviews
Prl Cmv Vectors, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prl-cmv+vector/prl+cmv+plasmid/pm40639948-317-5-10
Average 90 stars, based on 1 article reviews
prl-cmv vectors - by Bioz Stars,
2026-10
90/100 stars
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Plasmid Preparation:Article Title: Fads2b Plays a Dominant Role in ∆6/∆5 Desaturation Activities Compared with Fads2a in Common Carp ( Cyprinus carpio ) Article Snippet: Transient transfections were performed through lipofection using Lipofectamine 2000TM reagent (Invitrogen, Waltham, MA, USA) in the presence of reduced serum medium (Invitrogen, Waltham, MA, USA) according to the manufacturer’s instructions when EPC cells reached 90–95% confluency, 0.8 μg of Article Title: Identification and Functional Analysis of Known and New Mutations in the Transcription Factor KLF1 Linked with β-Thalassemia-like Phenotypes. Article Snippet: To normalize luciferase assays, 25 ng of pRL-CMV vector (Promega, Madison, WI, USA) coding for Renilla were co-transfected with 225 ng of pGL4 luc constructs. Article Title: SARS-CoV-2 ORF7a Mutation Found in BF.5 and BF.7 Sublineages Impacts Its Functions Article Snippet: Other plasmids used in this study included the pEGFP-N1 (Clonetech), pRL-CMV vector (E226A) acquired from Article Title: SARS-CoV-2 ORF7a mutation found in BF.5 and BF.7 sublineages impacts its functions Article Snippet: Other plasmids used in this study include the pEGFP-N1 (Clonetech) and Article Title: A Sox17 downstream gene Rasip1 is involved in the hematopoietic activity of intra-aortic hematopoietic clusters in the midgestation mouse embryo Article Snippet: First, the Article Title: PolyQ length-based molecular encoding of vocalization frequency in FOXP2 Article Snippet: Twenty-four hours after plating, the cells were co-transfected with three plasmid vectors, i.e., ( i ) the pcDNA4/HisMax plasmid, either empty (control condition) or encoding human FOXP2 ( Hs ), or one of its polyQ variants ( Cs , Ms , Rf , and ΔQ 1 Q 2 ); ( ii ) the pGL3-SPRX2 plasmid ; ( iii ) the Article Title: Transcriptional analysis of the human PAX9 promoter Article Snippet: 0.8 μg of pGL3 Luciferase Reporter Vectors – Basic Vector ( Article Title: A Sox17 downstream gene Rasip1 is involved in the hematopoietic activity of intra-aortic hematopoietic clusters in the midgestation mouse embryo. Article Snippet: First, the Transfection:Article Title: Fads2b Plays a Dominant Role in ∆6/∆5 Desaturation Activities Compared with Fads2a in Common Carp ( Cyprinus carpio ) Article Snippet: Transient transfections were performed through lipofection using Lipofectamine 2000TM reagent (Invitrogen, Waltham, MA, USA) in the presence of reduced serum medium (Invitrogen, Waltham, MA, USA) according to the manufacturer’s instructions when EPC cells reached 90–95% confluency, 0.8 μg of Article Title: Identification and Functional Analysis of Known and New Mutations in the Transcription Factor KLF1 Linked with β-Thalassemia-like Phenotypes. Article Snippet: To normalize luciferase assays, 25 ng of pRL-CMV vector (Promega, Madison, WI, USA) coding for Renilla were co-transfected with 225 ng of pGL4 luc constructs. Article Title: SARS-CoV-2 ORF7a Mutation Found in BF.5 and BF.7 Sublineages Impacts Its Functions Article Snippet: Other plasmids used in this study included the pEGFP-N1 (Clonetech), pRL-CMV vector (E226A) acquired from Article Title: SARS-CoV-2 ORF7a mutation found in BF.5 and BF.7 sublineages impacts its functions Article Snippet: Other plasmids used in this study include the pEGFP-N1 (Clonetech) and Article Title: A Sox17 downstream gene Rasip1 is involved in the hematopoietic activity of intra-aortic hematopoietic clusters in the midgestation mouse embryo Article Snippet: First, the Article Title: PolyQ length-based molecular encoding of vocalization frequency in FOXP2 Article Snippet: Twenty-four hours after plating, the cells were co-transfected with three plasmid vectors, i.e., ( i ) the pcDNA4/HisMax plasmid, either empty (control condition) or encoding human FOXP2 ( Hs ), or one of its polyQ variants ( Cs , Ms , Rf , and ΔQ 1 Q 2 ); ( ii ) the pGL3-SPRX2 plasmid ; ( iii ) the Article Title: Transcriptional analysis of the human PAX9 promoter Article Snippet: 0.8 μg of pGL3 Luciferase Reporter Vectors – Basic Vector ( Article Title: A Sox17 downstream gene Rasip1 is involved in the hematopoietic activity of intra-aortic hematopoietic clusters in the midgestation mouse embryo. Article Snippet: First, the Virus:Article Title: Fads2b Plays a Dominant Role in ∆6/∆5 Desaturation Activities Compared with Fads2a in Common Carp ( Cyprinus carpio ) Article Snippet: Transient transfections were performed through lipofection using Lipofectamine 2000TM reagent (Invitrogen, Waltham, MA, USA) in the presence of reduced serum medium (Invitrogen, Waltham, MA, USA) according to the manufacturer’s instructions when EPC cells reached 90–95% confluency, 0.8 μg of Article Title: Identification and Functional Analysis of Known and New Mutations in the Transcription Factor KLF1 Linked with β-Thalassemia-like Phenotypes. Article Snippet: To normalize luciferase assays, 25 ng of pRL-CMV vector (Promega, Madison, WI, USA) coding for Renilla were co-transfected with 225 ng of pGL4 luc constructs. Article Title: SARS-CoV-2 ORF7a Mutation Found in BF.5 and BF.7 Sublineages Impacts Its Functions Article Snippet: Other plasmids used in this study included the pEGFP-N1 (Clonetech), pRL-CMV vector (E226A) acquired from Article Title: SARS-CoV-2 ORF7a mutation found in BF.5 and BF.7 sublineages impacts its functions Article Snippet: Other plasmids used in this study include the pEGFP-N1 (Clonetech) and Article Title: A Sox17 downstream gene Rasip1 is involved in the hematopoietic activity of intra-aortic hematopoietic clusters in the midgestation mouse embryo Article Snippet: First, the Article Title: PolyQ length-based molecular encoding of vocalization frequency in FOXP2 Article Snippet: Twenty-four hours after plating, the cells were co-transfected with three plasmid vectors, i.e., ( i ) the pcDNA4/HisMax plasmid, either empty (control condition) or encoding human FOXP2 ( Hs ), or one of its polyQ variants ( Cs , Ms , Rf , and ΔQ 1 Q 2 ); ( ii ) the pGL3-SPRX2 plasmid ; ( iii ) the Article Title: Transcriptional analysis of the human PAX9 promoter Article Snippet: 0.8 μg of pGL3 Luciferase Reporter Vectors – Basic Vector ( Article Title: A Sox17 downstream gene Rasip1 is involved in the hematopoietic activity of intra-aortic hematopoietic clusters in the midgestation mouse embryo. Article Snippet: First, the Recombinant:Article Title: Fads2b Plays a Dominant Role in ∆6/∆5 Desaturation Activities Compared with Fads2a in Common Carp ( Cyprinus carpio ) Article Snippet: Transient transfections were performed through lipofection using Lipofectamine 2000TM reagent (Invitrogen, Waltham, MA, USA) in the presence of reduced serum medium (Invitrogen, Waltham, MA, USA) according to the manufacturer’s instructions when EPC cells reached 90–95% confluency, 0.8 μg of Article Title: Identification and Functional Analysis of Known and New Mutations in the Transcription Factor KLF1 Linked with β-Thalassemia-like Phenotypes. Article Snippet: To normalize luciferase assays, 25 ng of pRL-CMV vector (Promega, Madison, WI, USA) coding for Renilla were co-transfected with 225 ng of pGL4 luc constructs. Article Title: SARS-CoV-2 ORF7a Mutation Found in BF.5 and BF.7 Sublineages Impacts Its Functions Article Snippet: Other plasmids used in this study included the pEGFP-N1 (Clonetech), pRL-CMV vector (E226A) acquired from Article Title: SARS-CoV-2 ORF7a mutation found in BF.5 and BF.7 sublineages impacts its functions Article Snippet: Other plasmids used in this study include the pEGFP-N1 (Clonetech) and Article Title: A Sox17 downstream gene Rasip1 is involved in the hematopoietic activity of intra-aortic hematopoietic clusters in the midgestation mouse embryo Article Snippet: First, the Article Title: PolyQ length-based molecular encoding of vocalization frequency in FOXP2 Article Snippet: Twenty-four hours after plating, the cells were co-transfected with three plasmid vectors, i.e., ( i ) the pcDNA4/HisMax plasmid, either empty (control condition) or encoding human FOXP2 ( Hs ), or one of its polyQ variants ( Cs , Ms , Rf , and ΔQ 1 Q 2 ); ( ii ) the pGL3-SPRX2 plasmid ; ( iii ) the Article Title: Transcriptional analysis of the human PAX9 promoter Article Snippet: 0.8 μg of pGL3 Luciferase Reporter Vectors – Basic Vector ( Article Title: A Sox17 downstream gene Rasip1 is involved in the hematopoietic activity of intra-aortic hematopoietic clusters in the midgestation mouse embryo. Article Snippet: First, the Cell Culture:Article Title: Fads2b Plays a Dominant Role in ∆6/∆5 Desaturation Activities Compared with Fads2a in Common Carp ( Cyprinus carpio ) Article Snippet: Transient transfections were performed through lipofection using Lipofectamine 2000TM reagent (Invitrogen, Waltham, MA, USA) in the presence of reduced serum medium (Invitrogen, Waltham, MA, USA) according to the manufacturer’s instructions when EPC cells reached 90–95% confluency, 0.8 μg of Article Title: Identification and Functional Analysis of Known and New Mutations in the Transcription Factor KLF1 Linked with β-Thalassemia-like Phenotypes. Article Snippet: To normalize luciferase assays, 25 ng of pRL-CMV vector (Promega, Madison, WI, USA) coding for Renilla were co-transfected with 225 ng of pGL4 luc constructs. Article Title: SARS-CoV-2 ORF7a Mutation Found in BF.5 and BF.7 Sublineages Impacts Its Functions Article Snippet: Other plasmids used in this study included the pEGFP-N1 (Clonetech), pRL-CMV vector (E226A) acquired from Article Title: SARS-CoV-2 ORF7a mutation found in BF.5 and BF.7 sublineages impacts its functions Article Snippet: Other plasmids used in this study include the pEGFP-N1 (Clonetech) and Article Title: A Sox17 downstream gene Rasip1 is involved in the hematopoietic activity of intra-aortic hematopoietic clusters in the midgestation mouse embryo Article Snippet: First, the Article Title: PolyQ length-based molecular encoding of vocalization frequency in FOXP2 Article Snippet: Twenty-four hours after plating, the cells were co-transfected with three plasmid vectors, i.e., ( i ) the pcDNA4/HisMax plasmid, either empty (control condition) or encoding human FOXP2 ( Hs ), or one of its polyQ variants ( Cs , Ms , Rf , and ΔQ 1 Q 2 ); ( ii ) the pGL3-SPRX2 plasmid ; ( iii ) the Article Title: Transcriptional analysis of the human PAX9 promoter Article Snippet: 0.8 μg of pGL3 Luciferase Reporter Vectors – Basic Vector ( Article Title: A Sox17 downstream gene Rasip1 is involved in the hematopoietic activity of intra-aortic hematopoietic clusters in the midgestation mouse embryo. Article Snippet: First, the Saline:Article Title: Fads2b Plays a Dominant Role in ∆6/∆5 Desaturation Activities Compared with Fads2a in Common Carp ( Cyprinus carpio ) Article Snippet: Transient transfections were performed through lipofection using Lipofectamine 2000TM reagent (Invitrogen, Waltham, MA, USA) in the presence of reduced serum medium (Invitrogen, Waltham, MA, USA) according to the manufacturer’s instructions when EPC cells reached 90–95% confluency, 0.8 μg of Article Title: Identification and Functional Analysis of Known and New Mutations in the Transcription Factor KLF1 Linked with β-Thalassemia-like Phenotypes. Article Snippet: To normalize luciferase assays, 25 ng of pRL-CMV vector (Promega, Madison, WI, USA) coding for Renilla were co-transfected with 225 ng of pGL4 luc constructs. Article Title: SARS-CoV-2 ORF7a Mutation Found in BF.5 and BF.7 Sublineages Impacts Its Functions Article Snippet: Other plasmids used in this study included the pEGFP-N1 (Clonetech), pRL-CMV vector (E226A) acquired from Article Title: SARS-CoV-2 ORF7a mutation found in BF.5 and BF.7 sublineages impacts its functions Article Snippet: Other plasmids used in this study include the pEGFP-N1 (Clonetech) and Article Title: A Sox17 downstream gene Rasip1 is involved in the hematopoietic activity of intra-aortic hematopoietic clusters in the midgestation mouse embryo Article Snippet: First, the Article Title: PolyQ length-based molecular encoding of vocalization frequency in FOXP2 Article Snippet: Twenty-four hours after plating, the cells were co-transfected with three plasmid vectors, i.e., ( i ) the pcDNA4/HisMax plasmid, either empty (control condition) or encoding human FOXP2 ( Hs ), or one of its polyQ variants ( Cs , Ms , Rf , and ΔQ 1 Q 2 ); ( ii ) the pGL3-SPRX2 plasmid ; ( iii ) the Article Title: Transcriptional analysis of the human PAX9 promoter Article Snippet: 0.8 μg of pGL3 Luciferase Reporter Vectors – Basic Vector ( Article Title: A Sox17 downstream gene Rasip1 is involved in the hematopoietic activity of intra-aortic hematopoietic clusters in the midgestation mouse embryo. Article Snippet: First, the Luciferase:Article Title: Fads2b Plays a Dominant Role in ∆6/∆5 Desaturation Activities Compared with Fads2a in Common Carp ( Cyprinus carpio ) Article Snippet: Transient transfections were performed through lipofection using Lipofectamine 2000TM reagent (Invitrogen, Waltham, MA, USA) in the presence of reduced serum medium (Invitrogen, Waltham, MA, USA) according to the manufacturer’s instructions when EPC cells reached 90–95% confluency, 0.8 μg of Article Title: Identification and Functional Analysis of Known and New Mutations in the Transcription Factor KLF1 Linked with β-Thalassemia-like Phenotypes. Article Snippet: To normalize luciferase assays, 25 ng of pRL-CMV vector (Promega, Madison, WI, USA) coding for Renilla were co-transfected with 225 ng of pGL4 luc constructs. Article Title: SARS-CoV-2 ORF7a Mutation Found in BF.5 and BF.7 Sublineages Impacts Its Functions Article Snippet: Other plasmids used in this study included the pEGFP-N1 (Clonetech), pRL-CMV vector (E226A) acquired from Article Title: SARS-CoV-2 ORF7a mutation found in BF.5 and BF.7 sublineages impacts its functions Article Snippet: Other plasmids used in this study include the pEGFP-N1 (Clonetech) and Article Title: A Sox17 downstream gene Rasip1 is involved in the hematopoietic activity of intra-aortic hematopoietic clusters in the midgestation mouse embryo Article Snippet: First, the Article Title: PolyQ length-based molecular encoding of vocalization frequency in FOXP2 Article Snippet: Twenty-four hours after plating, the cells were co-transfected with three plasmid vectors, i.e., ( i ) the pcDNA4/HisMax plasmid, either empty (control condition) or encoding human FOXP2 ( Hs ), or one of its polyQ variants ( Cs , Ms , Rf , and ΔQ 1 Q 2 ); ( ii ) the pGL3-SPRX2 plasmid ; ( iii ) the Article Title: Transcriptional analysis of the human PAX9 promoter Article Snippet: 0.8 μg of pGL3 Luciferase Reporter Vectors – Basic Vector ( Article Title: A Sox17 downstream gene Rasip1 is involved in the hematopoietic activity of intra-aortic hematopoietic clusters in the midgestation mouse embryo. Article Snippet: First, the Genomic Sequencing:Article Title: Fads2b Plays a Dominant Role in ∆6/∆5 Desaturation Activities Compared with Fads2a in Common Carp ( Cyprinus carpio ) Article Snippet: Transient transfections were performed through lipofection using Lipofectamine 2000TM reagent (Invitrogen, Waltham, MA, USA) in the presence of reduced serum medium (Invitrogen, Waltham, MA, USA) according to the manufacturer’s instructions when EPC cells reached 90–95% confluency, 0.8 μg of Article Title: Identification and Functional Analysis of Known and New Mutations in the Transcription Factor KLF1 Linked with β-Thalassemia-like Phenotypes. Article Snippet: To normalize luciferase assays, 25 ng of pRL-CMV vector (Promega, Madison, WI, USA) coding for Renilla were co-transfected with 225 ng of pGL4 luc constructs. Article Title: SARS-CoV-2 ORF7a Mutation Found in BF.5 and BF.7 Sublineages Impacts Its Functions Article Snippet: Other plasmids used in this study included the pEGFP-N1 (Clonetech), pRL-CMV vector (E226A) acquired from Article Title: SARS-CoV-2 ORF7a mutation found in BF.5 and BF.7 sublineages impacts its functions Article Snippet: Other plasmids used in this study include the pEGFP-N1 (Clonetech) and Article Title: A Sox17 downstream gene Rasip1 is involved in the hematopoietic activity of intra-aortic hematopoietic clusters in the midgestation mouse embryo Article Snippet: First, the Article Title: PolyQ length-based molecular encoding of vocalization frequency in FOXP2 Article Snippet: Twenty-four hours after plating, the cells were co-transfected with three plasmid vectors, i.e., ( i ) the pcDNA4/HisMax plasmid, either empty (control condition) or encoding human FOXP2 ( Hs ), or one of its polyQ variants ( Cs , Ms , Rf , and ΔQ 1 Q 2 ); ( ii ) the pGL3-SPRX2 plasmid ; ( iii ) the Article Title: Transcriptional analysis of the human PAX9 promoter Article Snippet: 0.8 μg of pGL3 Luciferase Reporter Vectors – Basic Vector ( Article Title: A Sox17 downstream gene Rasip1 is involved in the hematopoietic activity of intra-aortic hematopoietic clusters in the midgestation mouse embryo. Article Snippet: First, the Micro-CT:Article Title: Fads2b Plays a Dominant Role in ∆6/∆5 Desaturation Activities Compared with Fads2a in Common Carp ( Cyprinus carpio ) Article Snippet: Transient transfections were performed through lipofection using Lipofectamine 2000TM reagent (Invitrogen, Waltham, MA, USA) in the presence of reduced serum medium (Invitrogen, Waltham, MA, USA) according to the manufacturer’s instructions when EPC cells reached 90–95% confluency, 0.8 μg of Article Title: Identification and Functional Analysis of Known and New Mutations in the Transcription Factor KLF1 Linked with β-Thalassemia-like Phenotypes. Article Snippet: To normalize luciferase assays, 25 ng of pRL-CMV vector (Promega, Madison, WI, USA) coding for Renilla were co-transfected with 225 ng of pGL4 luc constructs. Article Title: SARS-CoV-2 ORF7a Mutation Found in BF.5 and BF.7 Sublineages Impacts Its Functions Article Snippet: Other plasmids used in this study included the pEGFP-N1 (Clonetech), pRL-CMV vector (E226A) acquired from Article Title: SARS-CoV-2 ORF7a mutation found in BF.5 and BF.7 sublineages impacts its functions Article Snippet: Other plasmids used in this study include the pEGFP-N1 (Clonetech) and Article Title: A Sox17 downstream gene Rasip1 is involved in the hematopoietic activity of intra-aortic hematopoietic clusters in the midgestation mouse embryo Article Snippet: First, the Article Title: PolyQ length-based molecular encoding of vocalization frequency in FOXP2 Article Snippet: Twenty-four hours after plating, the cells were co-transfected with three plasmid vectors, i.e., ( i ) the pcDNA4/HisMax plasmid, either empty (control condition) or encoding human FOXP2 ( Hs ), or one of its polyQ variants ( Cs , Ms , Rf , and ΔQ 1 Q 2 ); ( ii ) the pGL3-SPRX2 plasmid ; ( iii ) the Article Title: Transcriptional analysis of the human PAX9 promoter Article Snippet: 0.8 μg of pGL3 Luciferase Reporter Vectors – Basic Vector ( Article Title: A Sox17 downstream gene Rasip1 is involved in the hematopoietic activity of intra-aortic hematopoietic clusters in the midgestation mouse embryo. Article Snippet: First, the Software:Article Title: Fads2b Plays a Dominant Role in ∆6/∆5 Desaturation Activities Compared with Fads2a in Common Carp ( Cyprinus carpio ) Article Snippet: Transient transfections were performed through lipofection using Lipofectamine 2000TM reagent (Invitrogen, Waltham, MA, USA) in the presence of reduced serum medium (Invitrogen, Waltham, MA, USA) according to the manufacturer’s instructions when EPC cells reached 90–95% confluency, 0.8 μg of Article Title: Identification and Functional Analysis of Known and New Mutations in the Transcription Factor KLF1 Linked with β-Thalassemia-like Phenotypes. Article Snippet: To normalize luciferase assays, 25 ng of pRL-CMV vector (Promega, Madison, WI, USA) coding for Renilla were co-transfected with 225 ng of pGL4 luc constructs. Article Title: SARS-CoV-2 ORF7a Mutation Found in BF.5 and BF.7 Sublineages Impacts Its Functions Article Snippet: Other plasmids used in this study included the pEGFP-N1 (Clonetech), pRL-CMV vector (E226A) acquired from Article Title: SARS-CoV-2 ORF7a mutation found in BF.5 and BF.7 sublineages impacts its functions Article Snippet: Other plasmids used in this study include the pEGFP-N1 (Clonetech) and Article Title: A Sox17 downstream gene Rasip1 is involved in the hematopoietic activity of intra-aortic hematopoietic clusters in the midgestation mouse embryo Article Snippet: First, the Article Title: PolyQ length-based molecular encoding of vocalization frequency in FOXP2 Article Snippet: Twenty-four hours after plating, the cells were co-transfected with three plasmid vectors, i.e., ( i ) the pcDNA4/HisMax plasmid, either empty (control condition) or encoding human FOXP2 ( Hs ), or one of its polyQ variants ( Cs , Ms , Rf , and ΔQ 1 Q 2 ); ( ii ) the pGL3-SPRX2 plasmid ; ( iii ) the Article Title: Transcriptional analysis of the human PAX9 promoter Article Snippet: 0.8 μg of pGL3 Luciferase Reporter Vectors – Basic Vector ( Article Title: A Sox17 downstream gene Rasip1 is involved in the hematopoietic activity of intra-aortic hematopoietic clusters in the midgestation mouse embryo. Article Snippet: First, the Microscopy:Article Title: Fads2b Plays a Dominant Role in ∆6/∆5 Desaturation Activities Compared with Fads2a in Common Carp ( Cyprinus carpio ) Article Snippet: Transient transfections were performed through lipofection using Lipofectamine 2000TM reagent (Invitrogen, Waltham, MA, USA) in the presence of reduced serum medium (Invitrogen, Waltham, MA, USA) according to the manufacturer’s instructions when EPC cells reached 90–95% confluency, 0.8 μg of Article Title: Identification and Functional Analysis of Known and New Mutations in the Transcription Factor KLF1 Linked with β-Thalassemia-like Phenotypes. Article Snippet: To normalize luciferase assays, 25 ng of pRL-CMV vector (Promega, Madison, WI, USA) coding for Renilla were co-transfected with 225 ng of pGL4 luc constructs. Article Title: SARS-CoV-2 ORF7a Mutation Found in BF.5 and BF.7 Sublineages Impacts Its Functions Article Snippet: Other plasmids used in this study included the pEGFP-N1 (Clonetech), pRL-CMV vector (E226A) acquired from Article Title: SARS-CoV-2 ORF7a mutation found in BF.5 and BF.7 sublineages impacts its functions Article Snippet: Other plasmids used in this study include the pEGFP-N1 (Clonetech) and Article Title: A Sox17 downstream gene Rasip1 is involved in the hematopoietic activity of intra-aortic hematopoietic clusters in the midgestation mouse embryo Article Snippet: First, the Article Title: PolyQ length-based molecular encoding of vocalization frequency in FOXP2 Article Snippet: Twenty-four hours after plating, the cells were co-transfected with three plasmid vectors, i.e., ( i ) the pcDNA4/HisMax plasmid, either empty (control condition) or encoding human FOXP2 ( Hs ), or one of its polyQ variants ( Cs , Ms , Rf , and ΔQ 1 Q 2 ); ( ii ) the pGL3-SPRX2 plasmid ; ( iii ) the Article Title: Transcriptional analysis of the human PAX9 promoter Article Snippet: 0.8 μg of pGL3 Luciferase Reporter Vectors – Basic Vector ( Article Title: A Sox17 downstream gene Rasip1 is involved in the hematopoietic activity of intra-aortic hematopoietic clusters in the midgestation mouse embryo. Article Snippet: First, the Fluorescence:Article Title: Fads2b Plays a Dominant Role in ∆6/∆5 Desaturation Activities Compared with Fads2a in Common Carp ( Cyprinus carpio ) Article Snippet: Transient transfections were performed through lipofection using Lipofectamine 2000TM reagent (Invitrogen, Waltham, MA, USA) in the presence of reduced serum medium (Invitrogen, Waltham, MA, USA) according to the manufacturer’s instructions when EPC cells reached 90–95% confluency, 0.8 μg of Article Title: Identification and Functional Analysis of Known and New Mutations in the Transcription Factor KLF1 Linked with β-Thalassemia-like Phenotypes. Article Snippet: To normalize luciferase assays, 25 ng of pRL-CMV vector (Promega, Madison, WI, USA) coding for Renilla were co-transfected with 225 ng of pGL4 luc constructs. Article Title: SARS-CoV-2 ORF7a Mutation Found in BF.5 and BF.7 Sublineages Impacts Its Functions Article Snippet: Other plasmids used in this study included the pEGFP-N1 (Clonetech), pRL-CMV vector (E226A) acquired from Article Title: SARS-CoV-2 ORF7a mutation found in BF.5 and BF.7 sublineages impacts its functions Article Snippet: Other plasmids used in this study include the pEGFP-N1 (Clonetech) and Article Title: A Sox17 downstream gene Rasip1 is involved in the hematopoietic activity of intra-aortic hematopoietic clusters in the midgestation mouse embryo Article Snippet: First, the Article Title: PolyQ length-based molecular encoding of vocalization frequency in FOXP2 Article Snippet: Twenty-four hours after plating, the cells were co-transfected with three plasmid vectors, i.e., ( i ) the pcDNA4/HisMax plasmid, either empty (control condition) or encoding human FOXP2 ( Hs ), or one of its polyQ variants ( Cs , Ms , Rf , and ΔQ 1 Q 2 ); ( ii ) the pGL3-SPRX2 plasmid ; ( iii ) the Article Title: Transcriptional analysis of the human PAX9 promoter Article Snippet: 0.8 μg of pGL3 Luciferase Reporter Vectors – Basic Vector ( Article Title: A Sox17 downstream gene Rasip1 is involved in the hematopoietic activity of intra-aortic hematopoietic clusters in the midgestation mouse embryo. Article Snippet: First, the Mutagenesis:Article Title: Fads2b Plays a Dominant Role in ∆6/∆5 Desaturation Activities Compared with Fads2a in Common Carp ( Cyprinus carpio ) Article Snippet: Transient transfections were performed through lipofection using Lipofectamine 2000TM reagent (Invitrogen, Waltham, MA, USA) in the presence of reduced serum medium (Invitrogen, Waltham, MA, USA) according to the manufacturer’s instructions when EPC cells reached 90–95% confluency, 0.8 μg of Article Title: Identification and Functional Analysis of Known and New Mutations in the Transcription Factor KLF1 Linked with β-Thalassemia-like Phenotypes. Article Snippet: To normalize luciferase assays, 25 ng of pRL-CMV vector (Promega, Madison, WI, USA) coding for Renilla were co-transfected with 225 ng of pGL4 luc constructs. Article Title: SARS-CoV-2 ORF7a Mutation Found in BF.5 and BF.7 Sublineages Impacts Its Functions Article Snippet: Other plasmids used in this study included the pEGFP-N1 (Clonetech), pRL-CMV vector (E226A) acquired from Article Title: SARS-CoV-2 ORF7a mutation found in BF.5 and BF.7 sublineages impacts its functions Article Snippet: Other plasmids used in this study include the pEGFP-N1 (Clonetech) and Article Title: A Sox17 downstream gene Rasip1 is involved in the hematopoietic activity of intra-aortic hematopoietic clusters in the midgestation mouse embryo Article Snippet: First, the Article Title: PolyQ length-based molecular encoding of vocalization frequency in FOXP2 Article Snippet: Twenty-four hours after plating, the cells were co-transfected with three plasmid vectors, i.e., ( i ) the pcDNA4/HisMax plasmid, either empty (control condition) or encoding human FOXP2 ( Hs ), or one of its polyQ variants ( Cs , Ms , Rf , and ΔQ 1 Q 2 ); ( ii ) the pGL3-SPRX2 plasmid ; ( iii ) the Article Title: Transcriptional analysis of the human PAX9 promoter Article Snippet: 0.8 μg of pGL3 Luciferase Reporter Vectors – Basic Vector ( Article Title: A Sox17 downstream gene Rasip1 is involved in the hematopoietic activity of intra-aortic hematopoietic clusters in the midgestation mouse embryo. Article Snippet: First, the Expressing:Article Title: Fads2b Plays a Dominant Role in ∆6/∆5 Desaturation Activities Compared with Fads2a in Common Carp ( Cyprinus carpio ) Article Snippet: Transient transfections were performed through lipofection using Lipofectamine 2000TM reagent (Invitrogen, Waltham, MA, USA) in the presence of reduced serum medium (Invitrogen, Waltham, MA, USA) according to the manufacturer’s instructions when EPC cells reached 90–95% confluency, 0.8 μg of Article Title: Identification and Functional Analysis of Known and New Mutations in the Transcription Factor KLF1 Linked with β-Thalassemia-like Phenotypes. Article Snippet: To normalize luciferase assays, 25 ng of pRL-CMV vector (Promega, Madison, WI, USA) coding for Renilla were co-transfected with 225 ng of pGL4 luc constructs. Article Title: SARS-CoV-2 ORF7a Mutation Found in BF.5 and BF.7 Sublineages Impacts Its Functions Article Snippet: Other plasmids used in this study included the pEGFP-N1 (Clonetech), pRL-CMV vector (E226A) acquired from Article Title: SARS-CoV-2 ORF7a mutation found in BF.5 and BF.7 sublineages impacts its functions Article Snippet: Other plasmids used in this study include the pEGFP-N1 (Clonetech) and Article Title: A Sox17 downstream gene Rasip1 is involved in the hematopoietic activity of intra-aortic hematopoietic clusters in the midgestation mouse embryo Article Snippet: First, the Article Title: PolyQ length-based molecular encoding of vocalization frequency in FOXP2 Article Snippet: Twenty-four hours after plating, the cells were co-transfected with three plasmid vectors, i.e., ( i ) the pcDNA4/HisMax plasmid, either empty (control condition) or encoding human FOXP2 ( Hs ), or one of its polyQ variants ( Cs , Ms , Rf , and ΔQ 1 Q 2 ); ( ii ) the pGL3-SPRX2 plasmid ; ( iii ) the Article Title: Transcriptional analysis of the human PAX9 promoter Article Snippet: 0.8 μg of pGL3 Luciferase Reporter Vectors – Basic Vector ( Article Title: A Sox17 downstream gene Rasip1 is involved in the hematopoietic activity of intra-aortic hematopoietic clusters in the midgestation mouse embryo. Article Snippet: First, the Reverse Transcription Polymerase Chain Reaction:Article Title: Fads2b Plays a Dominant Role in ∆6/∆5 Desaturation Activities Compared with Fads2a in Common Carp ( Cyprinus carpio ) Article Snippet: Transient transfections were performed through lipofection using Lipofectamine 2000TM reagent (Invitrogen, Waltham, MA, USA) in the presence of reduced serum medium (Invitrogen, Waltham, MA, USA) according to the manufacturer’s instructions when EPC cells reached 90–95% confluency, 0.8 μg of Article Title: Identification and Functional Analysis of Known and New Mutations in the Transcription Factor KLF1 Linked with β-Thalassemia-like Phenotypes. Article Snippet: To normalize luciferase assays, 25 ng of pRL-CMV vector (Promega, Madison, WI, USA) coding for Renilla were co-transfected with 225 ng of pGL4 luc constructs. Article Title: SARS-CoV-2 ORF7a Mutation Found in BF.5 and BF.7 Sublineages Impacts Its Functions Article Snippet: Other plasmids used in this study included the pEGFP-N1 (Clonetech), pRL-CMV vector (E226A) acquired from Article Title: SARS-CoV-2 ORF7a mutation found in BF.5 and BF.7 sublineages impacts its functions Article Snippet: Other plasmids used in this study include the pEGFP-N1 (Clonetech) and Article Title: A Sox17 downstream gene Rasip1 is involved in the hematopoietic activity of intra-aortic hematopoietic clusters in the midgestation mouse embryo Article Snippet: First, the Article Title: PolyQ length-based molecular encoding of vocalization frequency in FOXP2 Article Snippet: Twenty-four hours after plating, the cells were co-transfected with three plasmid vectors, i.e., ( i ) the pcDNA4/HisMax plasmid, either empty (control condition) or encoding human FOXP2 ( Hs ), or one of its polyQ variants ( Cs , Ms , Rf , and ΔQ 1 Q 2 ); ( ii ) the pGL3-SPRX2 plasmid ; ( iii ) the Article Title: Transcriptional analysis of the human PAX9 promoter Article Snippet: 0.8 μg of pGL3 Luciferase Reporter Vectors – Basic Vector ( Article Title: A Sox17 downstream gene Rasip1 is involved in the hematopoietic activity of intra-aortic hematopoietic clusters in the midgestation mouse embryo. Article Snippet: First, the Immunofluorescence:Article Title: Fads2b Plays a Dominant Role in ∆6/∆5 Desaturation Activities Compared with Fads2a in Common Carp ( Cyprinus carpio ) Article Snippet: Transient transfections were performed through lipofection using Lipofectamine 2000TM reagent (Invitrogen, Waltham, MA, USA) in the presence of reduced serum medium (Invitrogen, Waltham, MA, USA) according to the manufacturer’s instructions when EPC cells reached 90–95% confluency, 0.8 μg of Article Title: Identification and Functional Analysis of Known and New Mutations in the Transcription Factor KLF1 Linked with β-Thalassemia-like Phenotypes. Article Snippet: To normalize luciferase assays, 25 ng of pRL-CMV vector (Promega, Madison, WI, USA) coding for Renilla were co-transfected with 225 ng of pGL4 luc constructs. Article Title: SARS-CoV-2 ORF7a Mutation Found in BF.5 and BF.7 Sublineages Impacts Its Functions Article Snippet: Other plasmids used in this study included the pEGFP-N1 (Clonetech), pRL-CMV vector (E226A) acquired from Article Title: SARS-CoV-2 ORF7a mutation found in BF.5 and BF.7 sublineages impacts its functions Article Snippet: Other plasmids used in this study include the pEGFP-N1 (Clonetech) and Article Title: A Sox17 downstream gene Rasip1 is involved in the hematopoietic activity of intra-aortic hematopoietic clusters in the midgestation mouse embryo Article Snippet: First, the Article Title: PolyQ length-based molecular encoding of vocalization frequency in FOXP2 Article Snippet: Twenty-four hours after plating, the cells were co-transfected with three plasmid vectors, i.e., ( i ) the pcDNA4/HisMax plasmid, either empty (control condition) or encoding human FOXP2 ( Hs ), or one of its polyQ variants ( Cs , Ms , Rf , and ΔQ 1 Q 2 ); ( ii ) the pGL3-SPRX2 plasmid ; ( iii ) the Article Title: Transcriptional analysis of the human PAX9 promoter Article Snippet: 0.8 μg of pGL3 Luciferase Reporter Vectors – Basic Vector ( Article Title: A Sox17 downstream gene Rasip1 is involved in the hematopoietic activity of intra-aortic hematopoietic clusters in the midgestation mouse embryo. Article Snippet: First, the Staining:Article Title: Fads2b Plays a Dominant Role in ∆6/∆5 Desaturation Activities Compared with Fads2a in Common Carp ( Cyprinus carpio ) Article Snippet: Transient transfections were performed through lipofection using Lipofectamine 2000TM reagent (Invitrogen, Waltham, MA, USA) in the presence of reduced serum medium (Invitrogen, Waltham, MA, USA) according to the manufacturer’s instructions when EPC cells reached 90–95% confluency, 0.8 μg of Article Title: Identification and Functional Analysis of Known and New Mutations in the Transcription Factor KLF1 Linked with β-Thalassemia-like Phenotypes. Article Snippet: To normalize luciferase assays, 25 ng of pRL-CMV vector (Promega, Madison, WI, USA) coding for Renilla were co-transfected with 225 ng of pGL4 luc constructs. Article Title: SARS-CoV-2 ORF7a Mutation Found in BF.5 and BF.7 Sublineages Impacts Its Functions Article Snippet: Other plasmids used in this study included the pEGFP-N1 (Clonetech), pRL-CMV vector (E226A) acquired from Article Title: SARS-CoV-2 ORF7a mutation found in BF.5 and BF.7 sublineages impacts its functions Article Snippet: Other plasmids used in this study include the pEGFP-N1 (Clonetech) and Article Title: A Sox17 downstream gene Rasip1 is involved in the hematopoietic activity of intra-aortic hematopoietic clusters in the midgestation mouse embryo Article Snippet: First, the Article Title: PolyQ length-based molecular encoding of vocalization frequency in FOXP2 Article Snippet: Twenty-four hours after plating, the cells were co-transfected with three plasmid vectors, i.e., ( i ) the pcDNA4/HisMax plasmid, either empty (control condition) or encoding human FOXP2 ( Hs ), or one of its polyQ variants ( Cs , Ms , Rf , and ΔQ 1 Q 2 ); ( ii ) the pGL3-SPRX2 plasmid ; ( iii ) the Article Title: Transcriptional analysis of the human PAX9 promoter Article Snippet: 0.8 μg of pGL3 Luciferase Reporter Vectors – Basic Vector ( Article Title: A Sox17 downstream gene Rasip1 is involved in the hematopoietic activity of intra-aortic hematopoietic clusters in the midgestation mouse embryo. Article Snippet: First, the Binding Assay:Article Title: Fads2b Plays a Dominant Role in ∆6/∆5 Desaturation Activities Compared with Fads2a in Common Carp ( Cyprinus carpio ) Article Snippet: Transient transfections were performed through lipofection using Lipofectamine 2000TM reagent (Invitrogen, Waltham, MA, USA) in the presence of reduced serum medium (Invitrogen, Waltham, MA, USA) according to the manufacturer’s instructions when EPC cells reached 90–95% confluency, 0.8 μg of Article Title: Identification and Functional Analysis of Known and New Mutations in the Transcription Factor KLF1 Linked with β-Thalassemia-like Phenotypes. Article Snippet: To normalize luciferase assays, 25 ng of pRL-CMV vector (Promega, Madison, WI, USA) coding for Renilla were co-transfected with 225 ng of pGL4 luc constructs. Article Title: SARS-CoV-2 ORF7a Mutation Found in BF.5 and BF.7 Sublineages Impacts Its Functions Article Snippet: Other plasmids used in this study included the pEGFP-N1 (Clonetech), pRL-CMV vector (E226A) acquired from Article Title: SARS-CoV-2 ORF7a mutation found in BF.5 and BF.7 sublineages impacts its functions Article Snippet: Other plasmids used in this study include the pEGFP-N1 (Clonetech) and Article Title: A Sox17 downstream gene Rasip1 is involved in the hematopoietic activity of intra-aortic hematopoietic clusters in the midgestation mouse embryo Article Snippet: First, the Article Title: PolyQ length-based molecular encoding of vocalization frequency in FOXP2 Article Snippet: Twenty-four hours after plating, the cells were co-transfected with three plasmid vectors, i.e., ( i ) the pcDNA4/HisMax plasmid, either empty (control condition) or encoding human FOXP2 ( Hs ), or one of its polyQ variants ( Cs , Ms , Rf , and ΔQ 1 Q 2 ); ( ii ) the pGL3-SPRX2 plasmid ; ( iii ) the Article Title: Transcriptional analysis of the human PAX9 promoter Article Snippet: 0.8 μg of pGL3 Luciferase Reporter Vectors – Basic Vector ( Article Title: A Sox17 downstream gene Rasip1 is involved in the hematopoietic activity of intra-aortic hematopoietic clusters in the midgestation mouse embryo. Article Snippet: First, the Inhibition:Article Title: Fads2b Plays a Dominant Role in ∆6/∆5 Desaturation Activities Compared with Fads2a in Common Carp ( Cyprinus carpio ) Article Snippet: Transient transfections were performed through lipofection using Lipofectamine 2000TM reagent (Invitrogen, Waltham, MA, USA) in the presence of reduced serum medium (Invitrogen, Waltham, MA, USA) according to the manufacturer’s instructions when EPC cells reached 90–95% confluency, 0.8 μg of Article Title: Identification and Functional Analysis of Known and New Mutations in the Transcription Factor KLF1 Linked with β-Thalassemia-like Phenotypes. Article Snippet: To normalize luciferase assays, 25 ng of pRL-CMV vector (Promega, Madison, WI, USA) coding for Renilla were co-transfected with 225 ng of pGL4 luc constructs. Article Title: SARS-CoV-2 ORF7a Mutation Found in BF.5 and BF.7 Sublineages Impacts Its Functions Article Snippet: Other plasmids used in this study included the pEGFP-N1 (Clonetech), pRL-CMV vector (E226A) acquired from Article Title: SARS-CoV-2 ORF7a mutation found in BF.5 and BF.7 sublineages impacts its functions Article Snippet: Other plasmids used in this study include the pEGFP-N1 (Clonetech) and Article Title: A Sox17 downstream gene Rasip1 is involved in the hematopoietic activity of intra-aortic hematopoietic clusters in the midgestation mouse embryo Article Snippet: First, the Article Title: PolyQ length-based molecular encoding of vocalization frequency in FOXP2 Article Snippet: Twenty-four hours after plating, the cells were co-transfected with three plasmid vectors, i.e., ( i ) the pcDNA4/HisMax plasmid, either empty (control condition) or encoding human FOXP2 ( Hs ), or one of its polyQ variants ( Cs , Ms , Rf , and ΔQ 1 Q 2 ); ( ii ) the pGL3-SPRX2 plasmid ; ( iii ) the Article Title: Transcriptional analysis of the human PAX9 promoter Article Snippet: 0.8 μg of pGL3 Luciferase Reporter Vectors – Basic Vector ( Article Title: A Sox17 downstream gene Rasip1 is involved in the hematopoietic activity of intra-aortic hematopoietic clusters in the midgestation mouse embryo. Article Snippet: First, the Activation Assay:Article Title: Fads2b Plays a Dominant Role in ∆6/∆5 Desaturation Activities Compared with Fads2a in Common Carp ( Cyprinus carpio ) Article Snippet: Transient transfections were performed through lipofection using Lipofectamine 2000TM reagent (Invitrogen, Waltham, MA, USA) in the presence of reduced serum medium (Invitrogen, Waltham, MA, USA) according to the manufacturer’s instructions when EPC cells reached 90–95% confluency, 0.8 μg of Article Title: Identification and Functional Analysis of Known and New Mutations in the Transcription Factor KLF1 Linked with β-Thalassemia-like Phenotypes. Article Snippet: To normalize luciferase assays, 25 ng of pRL-CMV vector (Promega, Madison, WI, USA) coding for Renilla were co-transfected with 225 ng of pGL4 luc constructs. Article Title: SARS-CoV-2 ORF7a Mutation Found in BF.5 and BF.7 Sublineages Impacts Its Functions Article Snippet: Other plasmids used in this study included the pEGFP-N1 (Clonetech), pRL-CMV vector (E226A) acquired from Article Title: SARS-CoV-2 ORF7a mutation found in BF.5 and BF.7 sublineages impacts its functions Article Snippet: Other plasmids used in this study include the pEGFP-N1 (Clonetech) and Article Title: A Sox17 downstream gene Rasip1 is involved in the hematopoietic activity of intra-aortic hematopoietic clusters in the midgestation mouse embryo Article Snippet: First, the Article Title: PolyQ length-based molecular encoding of vocalization frequency in FOXP2 Article Snippet: Twenty-four hours after plating, the cells were co-transfected with three plasmid vectors, i.e., ( i ) the pcDNA4/HisMax plasmid, either empty (control condition) or encoding human FOXP2 ( Hs ), or one of its polyQ variants ( Cs , Ms , Rf , and ΔQ 1 Q 2 ); ( ii ) the pGL3-SPRX2 plasmid ; ( iii ) the Article Title: Transcriptional analysis of the human PAX9 promoter Article Snippet: 0.8 μg of pGL3 Luciferase Reporter Vectors – Basic Vector ( Article Title: A Sox17 downstream gene Rasip1 is involved in the hematopoietic activity of intra-aortic hematopoietic clusters in the midgestation mouse embryo. Article Snippet: First, the Derivative Assay:Article Title: Fads2b Plays a Dominant Role in ∆6/∆5 Desaturation Activities Compared with Fads2a in Common Carp ( Cyprinus carpio ) Article Snippet: Transient transfections were performed through lipofection using Lipofectamine 2000TM reagent (Invitrogen, Waltham, MA, USA) in the presence of reduced serum medium (Invitrogen, Waltham, MA, USA) according to the manufacturer’s instructions when EPC cells reached 90–95% confluency, 0.8 μg of Article Title: Identification and Functional Analysis of Known and New Mutations in the Transcription Factor KLF1 Linked with β-Thalassemia-like Phenotypes. Article Snippet: To normalize luciferase assays, 25 ng of pRL-CMV vector (Promega, Madison, WI, USA) coding for Renilla were co-transfected with 225 ng of pGL4 luc constructs. Article Title: SARS-CoV-2 ORF7a Mutation Found in BF.5 and BF.7 Sublineages Impacts Its Functions Article Snippet: Other plasmids used in this study included the pEGFP-N1 (Clonetech), pRL-CMV vector (E226A) acquired from Article Title: SARS-CoV-2 ORF7a mutation found in BF.5 and BF.7 sublineages impacts its functions Article Snippet: Other plasmids used in this study include the pEGFP-N1 (Clonetech) and Article Title: A Sox17 downstream gene Rasip1 is involved in the hematopoietic activity of intra-aortic hematopoietic clusters in the midgestation mouse embryo Article Snippet: First, the Article Title: PolyQ length-based molecular encoding of vocalization frequency in FOXP2 Article Snippet: Twenty-four hours after plating, the cells were co-transfected with three plasmid vectors, i.e., ( i ) the pcDNA4/HisMax plasmid, either empty (control condition) or encoding human FOXP2 ( Hs ), or one of its polyQ variants ( Cs , Ms , Rf , and ΔQ 1 Q 2 ); ( ii ) the pGL3-SPRX2 plasmid ; ( iii ) the Article Title: Transcriptional analysis of the human PAX9 promoter Article Snippet: 0.8 μg of pGL3 Luciferase Reporter Vectors – Basic Vector ( Article Title: A Sox17 downstream gene Rasip1 is involved in the hematopoietic activity of intra-aortic hematopoietic clusters in the midgestation mouse embryo. Article Snippet: First, the Electrophoresis:Article Title: Fads2b Plays a Dominant Role in ∆6/∆5 Desaturation Activities Compared with Fads2a in Common Carp ( Cyprinus carpio ) Article Snippet: Transient transfections were performed through lipofection using Lipofectamine 2000TM reagent (Invitrogen, Waltham, MA, USA) in the presence of reduced serum medium (Invitrogen, Waltham, MA, USA) according to the manufacturer’s instructions when EPC cells reached 90–95% confluency, 0.8 μg of Article Title: Identification and Functional Analysis of Known and New Mutations in the Transcription Factor KLF1 Linked with β-Thalassemia-like Phenotypes. Article Snippet: To normalize luciferase assays, 25 ng of pRL-CMV vector (Promega, Madison, WI, USA) coding for Renilla were co-transfected with 225 ng of pGL4 luc constructs. Article Title: SARS-CoV-2 ORF7a Mutation Found in BF.5 and BF.7 Sublineages Impacts Its Functions Article Snippet: Other plasmids used in this study included the pEGFP-N1 (Clonetech), pRL-CMV vector (E226A) acquired from Article Title: SARS-CoV-2 ORF7a mutation found in BF.5 and BF.7 sublineages impacts its functions Article Snippet: Other plasmids used in this study include the pEGFP-N1 (Clonetech) and Article Title: A Sox17 downstream gene Rasip1 is involved in the hematopoietic activity of intra-aortic hematopoietic clusters in the midgestation mouse embryo Article Snippet: First, the Article Title: PolyQ length-based molecular encoding of vocalization frequency in FOXP2 Article Snippet: Twenty-four hours after plating, the cells were co-transfected with three plasmid vectors, i.e., ( i ) the pcDNA4/HisMax plasmid, either empty (control condition) or encoding human FOXP2 ( Hs ), or one of its polyQ variants ( Cs , Ms , Rf , and ΔQ 1 Q 2 ); ( ii ) the pGL3-SPRX2 plasmid ; ( iii ) the Article Title: Transcriptional analysis of the human PAX9 promoter Article Snippet: 0.8 μg of pGL3 Luciferase Reporter Vectors – Basic Vector ( Article Title: A Sox17 downstream gene Rasip1 is involved in the hematopoietic activity of intra-aortic hematopoietic clusters in the midgestation mouse embryo. Article Snippet: First, the Immunoprecipitation:Article Title: Fads2b Plays a Dominant Role in ∆6/∆5 Desaturation Activities Compared with Fads2a in Common Carp ( Cyprinus carpio ) Article Snippet: Transient transfections were performed through lipofection using Lipofectamine 2000TM reagent (Invitrogen, Waltham, MA, USA) in the presence of reduced serum medium (Invitrogen, Waltham, MA, USA) according to the manufacturer’s instructions when EPC cells reached 90–95% confluency, 0.8 μg of Article Title: Identification and Functional Analysis of Known and New Mutations in the Transcription Factor KLF1 Linked with β-Thalassemia-like Phenotypes. Article Snippet: To normalize luciferase assays, 25 ng of pRL-CMV vector (Promega, Madison, WI, USA) coding for Renilla were co-transfected with 225 ng of pGL4 luc constructs. Article Title: SARS-CoV-2 ORF7a Mutation Found in BF.5 and BF.7 Sublineages Impacts Its Functions Article Snippet: Other plasmids used in this study included the pEGFP-N1 (Clonetech), pRL-CMV vector (E226A) acquired from Article Title: SARS-CoV-2 ORF7a mutation found in BF.5 and BF.7 sublineages impacts its functions Article Snippet: Other plasmids used in this study include the pEGFP-N1 (Clonetech) and Article Title: A Sox17 downstream gene Rasip1 is involved in the hematopoietic activity of intra-aortic hematopoietic clusters in the midgestation mouse embryo Article Snippet: First, the Article Title: PolyQ length-based molecular encoding of vocalization frequency in FOXP2 Article Snippet: Twenty-four hours after plating, the cells were co-transfected with three plasmid vectors, i.e., ( i ) the pcDNA4/HisMax plasmid, either empty (control condition) or encoding human FOXP2 ( Hs ), or one of its polyQ variants ( Cs , Ms , Rf , and ΔQ 1 Q 2 ); ( ii ) the pGL3-SPRX2 plasmid ; ( iii ) the Article Title: Transcriptional analysis of the human PAX9 promoter Article Snippet: 0.8 μg of pGL3 Luciferase Reporter Vectors – Basic Vector ( Article Title: A Sox17 downstream gene Rasip1 is involved in the hematopoietic activity of intra-aortic hematopoietic clusters in the midgestation mouse embryo. Article Snippet: First, the Construct:Article Title: Fads2b Plays a Dominant Role in ∆6/∆5 Desaturation Activities Compared with Fads2a in Common Carp ( Cyprinus carpio ) Article Snippet: Transient transfections were performed through lipofection using Lipofectamine 2000TM reagent (Invitrogen, Waltham, MA, USA) in the presence of reduced serum medium (Invitrogen, Waltham, MA, USA) according to the manufacturer’s instructions when EPC cells reached 90–95% confluency, 0.8 μg of Article Title: Identification and Functional Analysis of Known and New Mutations in the Transcription Factor KLF1 Linked with β-Thalassemia-like Phenotypes. Article Snippet: To normalize luciferase assays, 25 ng of pRL-CMV vector (Promega, Madison, WI, USA) coding for Renilla were co-transfected with 225 ng of pGL4 luc constructs. Article Title: SARS-CoV-2 ORF7a Mutation Found in BF.5 and BF.7 Sublineages Impacts Its Functions Article Snippet: Other plasmids used in this study included the pEGFP-N1 (Clonetech), pRL-CMV vector (E226A) acquired from Article Title: SARS-CoV-2 ORF7a mutation found in BF.5 and BF.7 sublineages impacts its functions Article Snippet: Other plasmids used in this study include the pEGFP-N1 (Clonetech) and Article Title: A Sox17 downstream gene Rasip1 is involved in the hematopoietic activity of intra-aortic hematopoietic clusters in the midgestation mouse embryo Article Snippet: First, the Article Title: PolyQ length-based molecular encoding of vocalization frequency in FOXP2 Article Snippet: Twenty-four hours after plating, the cells were co-transfected with three plasmid vectors, i.e., ( i ) the pcDNA4/HisMax plasmid, either empty (control condition) or encoding human FOXP2 ( Hs ), or one of its polyQ variants ( Cs , Ms , Rf , and ΔQ 1 Q 2 ); ( ii ) the pGL3-SPRX2 plasmid ; ( iii ) the Article Title: Transcriptional analysis of the human PAX9 promoter Article Snippet: 0.8 μg of pGL3 Luciferase Reporter Vectors – Basic Vector ( Article Title: A Sox17 downstream gene Rasip1 is involved in the hematopoietic activity of intra-aortic hematopoietic clusters in the midgestation mouse embryo. Article Snippet: First, the |